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High Performance Liquid Chromatography (HPLC)

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Training by Professional Experiences Training Center

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On-Site / Training

USD 4,250.00

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This course offers no-nonsense, results-oriented training that will help you improve your bottom-line performance. Come prepared to discuss your analytical problems and challenges with expert chromatographers. By enrolling in this course, you will learn to master state-of-the-art techniques and equipment in HPLC with practical, hands-on experience. In the course's hands-on laboratory sessions, you will work on a variety of experiments, and in the process, become familiar with HPLC hardware; column dimensions; mobile phase strength, flow rate, and temperature; and internal vs. external standard. By the end of the course, you will know how to develop a method for a complete unknown mixture.

Outline

HPLC Overview

- Introduction to HPLC

- HPLC Instrumentation Overview

- Detector Details

- Laboratory: HPLC Hardware

Separation Fundamentals and Mobile Phase

- Practical HPLC Theory

- Important HPLC Parameters

- The Mobile Phase

- Laboratory: Mobile Phase Strength, Flow Rate and Temperature

Separation Modes and Columns

- Reversed Phase

- Normal Phase

- Ion Exchange

- Size Exclusion

- Laboratory: pH Effects in Reversed Phase

- Laboratory: Column Dimensions

Quantitative Analysis and Troubleshooting

- Qualitative and Quantitative Analysis

- LC/MS

- Hardware Troubleshooting

- Separation Troubleshooting

- Laboratory: Internal vs. External Standard

- Hardware Troubleshooting

Gradients and Method Development

- Gradients

- Fundamentals of Method Development

- Laboratory: Develop a method for a compete unknown mixture

Special Offer

1 delegate – 15% discount

2 delegates – 20% discount

3 delegates – 2 with full amount + 1 free of charge

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PETC Training Company was established in response to the growing need for quality training to support the business community within your Company, Department or Organization by providing a high standard of Training Programs, Courses, Seminars, Workshops and Consultancy Services to employees in a very competitive business environment.

Following the latest trends and ideas in Technology and Management techniques, and with its in-depth knowledge of the business environment, PETC Training Company is able to meet the special requirements of both local and international business throughout the Middle East.

PETC Training & Consulting is an independent multinational firm with more than 25 years of experience in helping regional organizations develop their human capital in all fields of management. Since 1995, we have been entrusted by our clients to provide the most reliable training and consulting products and services in the region. Our innovative and diversified range of training programs and consulting services are designed and delivered by an ever-growing team of full time multilingual consultants experienced in providing solutions relevant to this part of the world and applicable to today’s business challenges. We pride ourselves on our track record in this regard and on our long-standing relationships with our clients, many of whom have been with us since we started business. ...

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Chemical Engineering